cy3 streptavidin incubation (Jackson Immuno)

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Jackson Immuno cy3 streptavidin incubation
Cy3 Streptavidin Incubation, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+cy3/Cy+3+Streptavidin/pmc13038874-73-39-43
Average 96 stars, based on 1770 article reviews

cy3 streptavidin incubation - by Bioz Stars, 2026-10

96/100 stars

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Article Title: The desmoglein 2 interactome in primary neonatal cardiomyocytes.
Article Snippet: Primary antibodies used for immunostaining were: anti-αE-catenin (1:100, Enzo Life Science, ALX-804-101-C100), anti-αT-catenin (1:100, rat, a kind gift from Jolanda Van Hengel, Ghent University), anti-β-catenin (1:100, Cell Signaling, D10A8), anti-I-afadin (1:500, Sigma, A0349), antiγ-catenin (1:100, Cell Signaling Technology, 2309), anti-connexin-43 (1:100, Proteintech, 15386-1-AP) and anti-plakophilin 2 (1:10, Progen, 651101).

Article Title: The desmoglein 2 interactome in primary neonatal cardiomyocytes
Article Snippet: Primary antibodies used for immunostaining were: anti-αE-catenin (1:100, Enzo Life Science, ALX-804-101-C100), anti-αT-catenin (1:100, rat, a kind gift from Jolanda Van Hengel, Ghent University), anti-β-catenin (1:100, Cell Signaling, D10A8), anti-I-afadin (1:500, Sigma, A0349), anti-γ-catenin (1:100, Cell Signaling Technology, 2309), anti-connexin-43 (1:100, Proteintech, 15386-1-AP) and anti-plakophilin 2 (1:10, Progen, 651101).

Immunofluorescence:

Article Title: Targeting the senescence-associated immune checkpoint GD3 ganglioside extends healthspan and blunt age-related diseases with sex-specific benefits
Article Snippet: Detection of primary antibodies involved the use of a biotinylated anti-mouse IgG (MOM kit) or anti-rabbit IgG (Jackson ImmunoResearch, ref# 111-065-144). .. For immunofluorescence, tissues were incubated with streptavidin-Cy3 (Jackson ImmunoResearch, ref# 016-160-084) for 1 hour at room temperature. .. Stained tissue sections were sequentially scanned using an HD Zeiss microscope, enabling imaging of entire sections.

Incubation:

Article Title: Single cell multiomic landscape reveals gene programs driving lipid droplet heterogeneity in hepatic steatosis.
Article Snippet: An additional blocking step with Avidin/Biotin Blocking Kit (SP-2001; Vector Laboratories) was performed according to manufacturer’s instructions for experiments using biotinylated anti-mouse secondary antibody (BA-9200, 1:100; Vector Laboratories). .. Next, samples were incubated for 30 minutes with streptavidin-Cy3 (016–160–084, 1:100; Jackson ImmunoResearch, West Grove, PA). .. Frozen tissue was sectioned into 10μm slices, fixed in 10% formalin and stained with BODIPY 493/503 (Invitrogen D3922) or Oil-Red O (Sigma O0625) and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma D9542) or hematoxylin, respectively.

Article Title: Single cell multiomic landscape reveals gene programs driving lipid droplet heterogeneity in hepatic steatosis
Article Snippet: An additional blocking step with Avidin/Biotin Blocking Kit (SP-2001; Vector Laboratories) was performed according to manufacturer’s instructions for experiments using biotinylated anti-mouse secondary antibody (BA-9200, 1:100; Vector Laboratories). .. Next, samples were incubated for 30 minutes with streptavidin-Cy3 (016–160–084, 1:100; Jackson ImmunoResearch, West Grove, PA). .. Finally, nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) at a 1:2500 concentration for 10 minutes and images were acquired using epifluorescence microscopes (Axio Observer and Axiovert 200; Carl Zeiss MicroImaging, Jena, Germany) or an inverted spinning-disk confocal microscopy system (Olympus IX81) as described previously.

Staining:

Article Title: Object location memories recruit distal CA1 and catecholaminergic inputs to proximodistal CA1
Article Snippet: A polyclonal rabbit antibody (1: 1000, Santa Cruz) was used for experiment 1 while a monoclonal rabbit antibody (1:5000, Cell signaling) was used for experiment 4. .. Following the primary antibody staining, slices were placed in biotinylated donkey anti-rabbit secondary antibody (1:500, Jackson ImmunoReseach) for sixty minutes at room temperature, followed by streptavidin-Cy3 (1:500, Jackson ImmunoResearch) for forty-five minutes). ..

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